grna lentiviral library (Addgene inc)
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94
Structured Review
Addgene inc
grna lentiviral library
Grna Lentiviral Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grna+lentiviral+library/Mouse+Improved+Genome-wide+Knockout+CRISPR+Library+v2+(Pooled+Library+%2367988)/bio_rxiv__64898__2026__03__18__712393-209-8-11
Average 94 stars, based on 48 article reviews
Grna Lentiviral Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grna+lentiviral+library/Mouse+Improved+Genome-wide+Knockout+CRISPR+Library+v2+(Pooled+Library+%2367988)/bio_rxiv__64898__2026__03__18__712393-209-8-11
Average 94 stars, based on 48 article reviews
grna lentiviral library - by Bioz Stars,
2026-11
94/100 stars
Images
1) Product Images from "PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity"
Article Title: PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity
Journal: bioRxiv
doi: 10.64898/2026.03.18.712393
Figure Legend Snippet: A. V-abl B cells expressing Flag-tagged Cas9 were infected with a BFP-expressing lentiviral gRNA library. BFP+ cells were sorted and treated with doxycycline for five days to induce Cas9 expression. Indicated inhibitors treatment was performed at IC90 for six days. Then, DNA was collected from the cells for sequencing and analysis using the MAGeCK pipeline. B. Scatter plot of total CRISPR genes Z-scores for Niraparib and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. C. Scatter plot of total CRISPR genes Z-scores for PDD0017273 and Olaparib IC90 treatments. Simple linear regression and Pearson correlation test were performed to determine R. D. Summary table of Pearson R coefficient for linear regression between cell lines sensitivity to PDD, olaparib, nirabarib and talazoparib. Depmap sensitivity score (PRISM repurposing data) of 536 cell lines to the indicated compound. Simple linear regression and Pearson correlation test were performed to determine R. Colored scaled is represented on the right and R coefficients are written in white. E. Heatmap representing z-score (color range) and False Discovery Rate FDR (size) for the genes targeting indicated in rows, in cells challenged with inhibitor indicated above, in columns. Gene implicated in homologous recombination are displayed. F. CRISPR screen z-scores ranking of co-essential genes with PDD00017273 treatment. SSBR related genes are shown in red, ADP-ribosylation related genes are shown in dark blue, HR related genes are shown in light grey.
Techniques Used: Expressing, Infection, Sequencing, CRISPR, Homologous Recombination
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Genome Wide:Article Title: Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-γ-signaling mutant cancer cells Article Snippet: .. Genome-wide Article Title: Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-γ-signaling mutant cancer cells. Article Snippet: For lentivirus packaging, Two hundred and ninetythree T cells were cultured in complete DMEM without antibiotics at 50–70% confluence in a 6-well plate and were then transfected with lipofectamine particles containing the target vector, psPAX2 (Addgene, #12260) and pMD2.G (Addgene: #12259) packaging vector (Addgene, #12260). .. Genome-wide Infection:Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion. Article Snippet: Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 μg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich, F3165). .. Once a suitable single clone was chosen, it was expanded and 100 million cells were infected with a BFP-expressing Article Title: PARG inhibition sequesters nuclear PAR-binding proteins, including XRCC1 and its partners, into nuclear condensates to elicit cytotoxicity Article Snippet: A single clone was selected for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 μg/ml for 3 days) and expanded. .. 100 million cells were infected with a BFP-expressing Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion Article Snippet: Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 μg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich, F3165). .. Once a suitable single clone was chosen, it was expanded and 100 million cells were infected with a BFP-expressing Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion Article Snippet: Single clones were generated and screened for high SpCas9 inducibility after doxycycline treatment (Sigma Aldrich, D9891, 3 μg/ml for 3 days) via Western Blot and flow cytometry after intracellular staining using anti-Flag antibody (Sigma Aldrich, F3165). .. Once a suitable single clone was chosen, it was expanded and 100 million cells were infected with a BFP-expressing |